Novel pathway of cypermethrin biodegradation in a Bacillus sp. strain SG2 isolated from cypermethrin-contaminated agriculture field
نویسندگان
چکیده
Pesticides belonging to pyrethroid group are widely used in agricultural fields to check pest infestation in different crops for enhanced food production. In spite of beneficial effects, non-judicious use of pesticides imposes harmful effect on human health as their residues reach different food materials and ground water via leaching, percolation and bioaccumulation. Looking into the potential of microbial degradation of toxic compounds under natural environment, a cypermethrin-degrading Bacillus sp. was isolated from pesticide-contaminated soil of a rice field of Distt. Udham Singh Nagar, Uttarakhand, India. The bacteria degraded the compound up to 81.6 % within 15 days under standard growth conditions (temperature 32 °C pH 7 and shaking at 116 rpm) in minimal medium. Analysis of intermediate compounds of biodegraded cypermethrin revealed that the bacteria opted a new pathway for cypermethrin degradation. GC-MS analysis of biodegraded cypermethrin showed the presence of 4-propylbenzoate, 4-propylbenzaldehyde, phenol M-tert-butyl and 1-dodecanol, etc. which was not reported earlier in cypermethrin metabolism; hence a novel biodegradation pathway of cypermethrin with Bacillus sp. strain SG2 is proposed in this study.
منابع مشابه
Differential expression and characterization of cypermethrin-degrading potential proteins in Bacillus thuringiensis strain, SG4
A cypermethrin-degrading bacterium (SG4) was isolated from the pesticide-contaminated soil in the agricultural field of the crop research centre of the University, and characterized as Bacillus thuringiensis strain, SG4. The bacterium degraded 78.9 % of cypermethrin (50 ppm) in 15 days when grown in a minimal medium. To understand the functional proteins of cypermethrin degradation in Bacillus ...
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